- ベストアンサー
形質転換？の語句で質問です
ある酵素を分泌する菌に対する形質転換の論文だと 思うのですが、その実験方法欄の冒頭で躓きました。 使用菌株について書いてあるのでしょうが、 どれが遺伝子で、とか全く判りません。 何か規則性があると聞いた気がしますが、 なにを意味しているのか判りません。 タイトルは 「The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation.」 です。 recA1,endA1,Nal^r,supE44,relA1 Δ(lacproAB)/F'・・・ などの、どれが何を表しているのか、 教えてください。 この手の話は初めてで、 どれから触っていいのかも判りません・・・。 Bacterial strains. Escherichia coli JM109 [recAl endA1 gyrA96 (Nalr) thi hsdRl7 (rk- Mk-) supE44 relA1 mcrA Δ(lacproAB)/F'(traD36 lacIq lacZ ΔM15 proA+B+)] was the host strain used throughout the construction of various recombinant plasmids, and E. coli strains BW313 {HfrKL16PO/45[lysA(61-62)], dut-1 ung-1 thi-1 relA4}, BMH71-18 [A(lacproAB) thi supE mutS 215::TnlO (Tetr)/F'(traD36proAB+ lacIq lacZ AM15)], and MV1184 [ara Δ(lac-proAB) rpsL thi (f8OlacZ ΔM15) (srl-recA)306::TnlO (Tetr)/FI(traD36proAB+ lacIq lacZ AM15)] were used in the oligonucleotide-directed mutagenesis. Intact and modified chitinases were produced in E. coli HB101 [supE44 hsdS 20(rB- mB) recA13 ara-14proA2 lacYl lalK2 rpsL20 xyl-5 mtl-l]. All strains carrying recombinant plasmids were grown in L broth medium containing 100 ,ug of ampicillin per ml.
- みんなの回答 （3）
- 専門家の回答
質問者が選んだベストアンサー
その他の回答 (2)
- blackdragon
- ベストアンサー率35% (428/1222)
- acroteria
- ベストアンサー率14% (7/49)
関連するQ&A
- XL1-Blueの遺伝子型について
XL1-Blue（SupE44,hsdR17,recA1,endA1,GyrA46,thi,relA1,lac-F'[proAB+,laclq,lacZΔ15,Tn10(tetr)]の遺伝子型の読み方について知りたいのですがほとんど意味が分かりません。どのように調べていけば性質は分かるでしょうか？
- ベストアンサー
- 生物学
- 制限酵素についての質問です。
今大学で微生物の研究をしているのですが、 どうしてもわからないことがあって、どうか教えていただきたいです。 一か所だけ制限酵素Clalの認識部位があるプラスミドを大腸菌Ａ株から抽出し、Clalで切断しようとしたら全く切断されませんでした。 同じプラスミドを大腸菌Ｂ株から抽出したところClalで切断することができました。 なぜ認識部位を持っているのに切断することができなかったのでしょうか？? よろしければ教えてください。 Ａ株：F'traD36 proA+B+laclq △(lacZ)M15/ △(lac-proAB) gyrA96 recA1 relA1 thi hsdR1 Ｂ株：ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 mcrB1 hsdR2
- ベストアンサー
- 生物学
- すいません、和訳お願いしたいです。
Jensen and coworkers traced the annual spring outbreaks of earthy-musty odor in tap water derived from a major Canadian river to actinomycetes, which were introduced into these surface waters during snowmelt and runoff. Klausen et al. concluded that actinomycetes were responsible for low concentrations of geosmin and 2-MIB in streams flowing past trout breeding aquaculture operations, because isolated strains of Streptomyces from these habitats were able to synthesize geosmin and 2-MIB, while cyanobacteria were not present in large numbers.
- ベストアンサー
- 英語
- 要約のアドバイスをお願いします
Spices have a long and surprising history. They ware first used in China about 5000 years ago, mostly for medical reasons. News of their medicinal properties and their flavors spread rapidly. Eventually, a huge spice market grew up around the Mediterranean coastal areas. In ancient Rome, spices were also used in place of money. For example, peppercorns were a preferred form of payment. They were convenient because they were small and they weren’t as heavy as metal coins. There was such a high demand for pepper that its trading value was high. A pound of pepper could buy several sheep. Only the richest Romans could afford to season their food with pepper, so it was a sort of status symbol. Most people couldn’t afford to use their pepper for cooking. Over the centuries, people mainly used spices to preserve foods and to cover up the taste of foods that were rotten or diseased. Spices were so important for those reasons that they indirectly led to the exploration of the world. In the 15th and 16th centuries, European explorers set out to find new ways to get them. Explorers like Columbus discovered new lands as a result of their search for new spice trade routes. In this way, the story of spices is quite fascinating. を80～90語で要約しないといけないのですが、どこをどう要約すればいいのか全然分かりません…。アドバイスをぜひお願いします。 自分なりにやってみたものです ↓ The history of spices is long and full of surprising facts. In China, spices were mainly used for medical reasons about 5000 years ago. They were also used instead of money in ancient Rome because they were very convenient that they were small and light. Moreover, they have effects of preserving foods and giving the taste of foods. Thus spices were used in various ways and they were one of the necessaries of peoples lives.
- ベストアンサー
- 英語
- 英文翻訳をお願いします。
The new system was used to calculate losses since August 1914, which took several months but the system had become established by February 1916. The états numériques des pertes were used to calculate casualty figures published in the Journal Officiel, the French Official History and other publications. The German armies compiled Verlustlisten every ten days, which were published by the Reichsarchiv in the deutsches Jahrbuch of 1924–1925. German medical units kept detailed records of medical treatment at the front and in hospital and in 1923, the Zentral Nachweiseamt published an amended edition of the lists produced during the war, incorporating medical service data not in the Verlustlisten. Monthly figures of wounded and ill servicemen that were treated were published in 1934 in the Sanitätsbericht.
- ベストアンサー
- 英語
- 和訳をお願いします。
長文で恐縮ですが、ニュアンスがわかる程度で結構ですので和訳をお願致します。 It has been noted throughout the temperate zones of the southern hemisphere, which are now passing out of their winter season, that when pandemic H1N1 began to circulate, the relative importance of seasonal strains, represented by H3N2 in nearly all countries, rapidly diminished and pandemic H1N1 became the dominant strain. Some seasonal H1N1 strains were reported but were even less common than seasonal H3N2.
- 締切済み
- 英語
- 英語論文についてです。
今、英語の論文を読んでいるのですが、訳せないところがあります。 As illustrated in Fig. 3C, site directed mutagenesis of either site 1 (S1) or site 2 (S2) resulted in a moderate but significant (P<0.0001) decrease in the expression of sseL::lacZ. Additionally, an sseL manipulated promoter containing both mutations (S1+S2) demonstrated accumulative reduction in the expression of sseL::lacZ by more than 2-fold (P<0.0001), indicating that the S1 and S2 sites are required for optimal expression of sseL, possibly due to their role as PhoP binding sites. To further examine this idea and to rule out the possibility that these positions are used as SsrB binding sites, we analyzed the expression of an altered sseL promoter harboring both mutations in a phoP ssrB background complemented with PhoP or SsrB, provided in trans. In the presence of SsrB, this mutated promoter was readily able to induce sseL::lacZ expression, suggesting that SsrB does not bind to these sites. In contrast, providing PhoP in trans did not induce sseL::lacZ expression from the modified promoter (Fig. 3D), supporting the function of S1 and S2 loci as potential PhoP binding sites. 少し長いですが、どなたか訳せる方、よろしくお願いします。 私事ながら時間がないのです！！助けてください！！
- 締切済み
- 英語
- when the United States jo
when the United States joined World war II in 1941 ,the country found it necessary to send soldiers and supplies across the Atlantic Ocean to Europe. Many of the ships it used, however, were sunk by German submarines. Many of the ships it used のit usedは何を意味しているのでしょうか？おそらくto send soldiers and supplies のことだと思うのですが、文法的にもよく分かりません。何か省略されているのでしょうか？
- ベストアンサー
- 英語
- 日本語訳をお願いいたします。
In The World Crisis, Winston Churchill used figures from French parliamentary records of 1920, to give French casualties from 5 August to 5 September 1914 of 329,000 killed, wounded and missing, German casualties from August to November of 677,440 men and British casualties in August and September of 29,598 men. By the end of August, the French Army had suffered 75,000 dead, of whom 27,000 were killed on 22 August. French casualties for the first month of the war were 260,000, of which 140,000 occurred during the last four days of the Battle of the Frontiers. In 2009, Herwig recorded that the casualties in the 6th Army in August were 34,598, with 11,476 men killed and 28,957 in September with 6,687 men killed. The 7th Army had 32,054 casualties in August, with 10,328 men killed and 31,887 casualties in September with 10,384 men killed. In the 1st Army in August there were 19,980 casualties including 2,863 men killed and in the 2nd Army 26,222 casualties. In the last ten days of August, the 1st Army had 9,644 casualties and the 2nd Army had losses of 15,693 men. Herwig wrote that the French army did not publish formal casualty lists but that the Official History Les armées françaises dans la grande guerre gave losses of 206,515 men for August and 213,445 for September. During the battle, French casualties were c. 260,000 men, of whom c. 75,000 men were killed.
- ベストアンサー
- 英語
お礼
解答ありがとうございます。 Δ～とか、数式モドキの表現が判りませんが、 まだ調べなおしてみます。